A | A microdevice containing interconnected cell culture microchambers was used to develop a multi-organ model that integrated microfluidic culture of intestinal epithelial cells, hepatocytes and breast cancer cells to simulate absorption, metabolism and activity of anticancer drugs. b | A micro cell culture analogue (μCCA) was created by linking together three interconnected microfluidic cell chambers representing a colon tumour, the bone marrow and liver with proportional physiological scaling to develop a more realistic physiological model of drug metabolism and anticancer activity. Culture media recirculates through the three inline chambers and an external reservoir, mimicking blood circulation and its residence times in the modelled organs. PBPK, physiologically based pharmacokinetic.