Figure 1.
Drosophila Wall Following Behavior Is Reduced by Diazepam
(A) Flies in a glass-topped arena walk on all interior surfaces.
(B) Tracking data from a 10-min experiment reveal that flies mainly walk in the perimeter of the arena.
(C) Flies fed with diazepam had decreased WAFO compared with controls (g1 = −0.32, g2 = −8.0, g3 = −0.67, g4 = −0.83, n = 40, 40). Fly WAFO was measured as mean distance from center in millimeters. Dots indicate the mean distance from center for individual flies; horizontal line indicates the mean distance from center (mm). p values determined by Mann-Whitney U. The lower axis represents the effect size in Hedges’ g with 95% CI. Green circles and asterisk (∗) mark a statistically significant (p < 0.05) decrease in behavior.
(D) Standardized mean effect sizes of diazepam effects on rodent anxiety (−0.85 g [95 CI −0.74, −0.96]) and fly WAFO (−0.83 g [95 CI −0.42, −0.91]) have comparable magnitudes.
See also Figure S1.