Figure 2.

mbt Promotes AJ Morphogenesis Independently from Baz

(A–D) mbt^P1 mutant, lacking GFP (blue; A), Arm (red; B), Baz (green; C), and merge (D). The scale bars represent 2 microns.

(E) Mean length of Arm cortical domain in wild-type and mbt^P1 mutants.

(F) Mean pixel intensity of Arm in wild-type and mbt^P1. In (E) and (F), n = 202 (in four wild-type retinas) and n = 460 (in four mbt^P1 retinas).

(G) Mean length of Baz cortical domain in wild-type and mbt^P1 mutants.

(H) Mean pixel intensity of Baz in wild-type and mbt^P1. In both (G) and (H), n = 99 (wild-type) and n = 107 (mbt^P1), with measurements taken from five independent mbt^P1 mosaic retina. In (E)–(H), columns represent mean and error bars represent the SEM of each dataset. Statistical significance was determined using an unpaired two-tailed Student’s t test.

(I and J) Electron microscopy (I) on a wild-type ommatidium and (J) on the poorly developed apical membranes of an mbt^P1 adult ommatidium. Ectopic AJ domains are boxed and sub-apical membranes in green. The scale bar represents 2 μm.

(K–N) baz⁴, mbt^P1 mutant lacking GFP (blue; K), Arm (green; L), aPKC (red; M), and merge (N). Asterisks highlight mutant cells. A tilde marks a wild-type cell. The scale bars represent 4 μm.

(O) FRAP on E-cadherin::GFP in wild-type or mbt^P1. Mean normalized fluorescence intensity in wild-type (gray; n = 18 from two individuals) and mbt^P1 (pink; n = 15 from three individuals) is shown; error bars represent SEM. Fluorescence recovery curves of E-cad::GFP after photo-bleaching in wild-type (black) and mbt^P1 (red) are shown.

(P) Mobile fraction of E-cadherin::GFP in a wild-type (black) or mbt^P1 (red) background. The p value was calculated with an unpaired two-tailed Student’s t test with Welch’s correction.