Figure S5.

Tryptophan-Derived Metabolites Restrict Root Colonization by C. tofieldiae, Related to Figure 5

To measure fungal biomass by qPCR, 500 ng RNA from infected plants was used to amplify the C. tofieldiae (Ct) actin fragment (CtACT) and RNA amounts were normalized to plant actin (AT3G18780).

(A) Under high Pi conditions at 4 dpi, Ct biomass was significantly higher in cyp79B2 cyp79B3 double mutants compared with Col-0 wild-type plants and the other tested plant defense mutants (Col-0 versus cyp79B2 cyp79B3, two-tailed t test, p < 0.05).

(B) Quantification of Ct biomass in roots of Col-0 and ein2 pad4 sid2 dde2 quadruple mutant plants grown under high and low Pi conditions at 24 dpi. Under both conditions, fungal biomass in ein2 pad4 sid2 dde2 plants was not significantly higher than in Col-0 wild-type plants.

(C) Inoculation of Ct onto the roots of cyp79B2 cyp79B3 mutant plants severely inhibited plant growth under high Pi conditions. The photograph was taken at 10 dpi.