Table 2.

Percentage of buffalo sperm cells with intact chromatin detected by acridine orange test in semen from four buffalo bulls (mean ± SE)

Bull number	Fresh	Frozen
1	99.67 ± 0.33	98.33 ± 0.88a
2	99.50 ± 0.28	97.50 ± 0.29ab
3	99.00 ± 0.58	97.00 ± 0.58ab
4	98.83 ± 0.44	96.00 ± 0.58b
Total means	99.25 ± 0.21**	97.21 ± 0.37

Values with different superscripts within the same column differ significantly (P<0.05).

^** P<0.001 (t-test) between fresh and frozen semen