Table 1.

Primers and sequences used for nested PCR

Snounou PCR
Primary	rPLU6	5′TTAAAATTGTTGCAGTTAAAACG3′
	rPLU5new	5′CYTGTTGTTGCCTTAAACTTC3′
P. falciparum	rFAL1	5′TTAAACTGGTTTGGGAAAACCAAATATATT3′
	rFAL2	5′ACACAATAGACTCAATCATGACTACCCGTC3′
P. malariae	rMAL1	5′ATAACATAGTTGTACGTTAAGAATAACCGC3′
	rMAL2	5′AAAATTCCCATGCATAAAAAATTATACAAA3′
P. vivax	rVIV1	5′CGCTTCTAGCTTAATCCACATAA3′
	rVIV2	5′ACTTCCAAGCCGAAGCAAAGAAA 3′
MSP2 PCR
Primary	S2-Fw	5′GAA GGT AAT TAA AAC ATT GTC3′
	S3-REv	5′GAG GGA TGT TGC TGC TCC ACA G3′
Nested	S1Tail	5′GCT TAT AAT ATG AGT ATA AGG AGA A3′
	M5	5′-6FAM-GCA TTG CCA GAA CTT GAA3′
	N5	5′-VIC-CTG AAG AGG TAC TGG TAG A3′