| First step: | 2:00 | 94°C | (initial denaturation) |
| 30 cycles: | 0:30 | 94°C | (denaturation) |
| 0:30 | 55°C | (annealing) | |
| 3:00 | 72°C | (extension) | |
| Final step | 10:00 | 72°C | (final extension) |
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