| First step: | 2:00 | 95ºC | (initial denaturation) |
| 30 cycles: | 0:20 | 95ºC | (denaturation) |
| 0:20 | PCR primer Tm - 5ºC | (annealing) | |
| ≥0:30 | 72ºC | (extension 30 s per kb) | |
| Final step: | 3:00 | 72ºC | (final extension) |
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