Figure 6.

PR deficiency leads to decreased splenic T_REG abundance in 10 mo.-old female Nba2 mice. Representative FACS plots show gating scheme used to sort CD3⁺CD4⁺ cells into T_FH cells and their TF_REG, and T_FH1 subsets (A), and non-T_FH CD4⁺ T cells into their T_REG and T_H1 subsets (B); T_FH = CD3⁺CD4⁺CXCR5⁺PD-1⁺; TF_REG = CD3⁺CD4⁺CXCR5⁺PD-1⁺FoxP3⁺; T_FH1 = CD3⁺CD4⁺CXCR5⁺PD-1⁺Tbet⁺; non-T_FH = CD3⁺CD4⁺CXCR5⁻PD-1⁻; T_REG = CD3⁺CD4⁺CXCR5⁻PD-1⁻FoxP3⁺; T_H1 = CD3⁺CD4⁺CXCR5⁻PD-1⁻Tbet⁺. (C – H) Shown are means (lines) and individual values (symbols) for splenic abundance of T_FH cells (C) and their TF_REG (D) T_FH1 (E) subsets, and non-T_FH CD4⁺ T cells (F) and their T_REG (G) and T_H1 (H) subsets. *, p < 0.05, **, p < 0.01, unpaired t test.