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. Author manuscript; available in PMC: 2016 May 30.
Published in final edited form as: Nature. 2015 Nov 30;528(7583):510–516. doi: 10.1038/nature15729

Extended Data Figure 5.

Extended Data Figure 5

Western blot detection of ΔF508 CFTR upon RNAi of interactors. ΔF508 CFTR was detected 48 – 72 h after lentiviral shRNA infection using the 3G11 antibody or 24.1 antibody (lowest left panel). Rescue is indicated by appearance of band C. Detection of β-actin served as loading control. Samples on the same blot represent parallel infections. Samples in the lower three left panels were lysed initially in TNI buffer, whereas samples in the other panels were lysed directly in 2× Laemmli sample buffer as described in Materials and Methods. Scr: scrambled non-target shRNA.