Extended Data Figure 7.

ΔF508 CFTR detection in primary bronchial epithelial cells upon RNAi of key interactors. a. Quantification of the ΔF508 CFTR ion channel activity (as fold change of the ΔI_sc relative to non-target shRNA) in comparison to the ratio of band C to band A/B in primary CF patient or healthy donor (wt) cells. b. Representative trace of forskolin (10 µM, F) and genistein (50 µM, G) activated, wt CFTR short circuit current (I_sc) in a 30 d ALI culture from a healthy donor. CFTR inhibitor 172 (I) indicates specificity of the measured I_sc for CFTR. c. Western blot of 28–30 d old primary human bronchial epithelial snapwell cultures from CF patients (DHBE) indicates formation of band C after specific knockdown of PABPC1, YBX1, PTBP1, TRIM21, PTPLAD1 and SURF4 with different shRNAs. Tubulin, β-actin or Na⁺/K⁺⁻ATPase was used as a loading control. Knockdown of PABPC1 and PTPLAD1 was verified by Western blotting with the respective antibodies. NT sh: non-target shRNA.