a, ELISA for binding of CFH and recombinantly expressed CFH fragments to coated BSA (white bars) or MAA-BSA (black bars). The length of CFH fragments is indicated by schematic representations with each circle depicting one SCR. Values are mean ± s.d. RLU per 100 ms of triplicate determinations. b, ELISA for binding of purified CFH variant Y402 and CFH variant H402 (both at 1 μg ml−1) to coated MAA-BSA. Values are mean ± s.d. RLU per 100 ms of triplicate determinations. c, ELISA for binding of plasma CFH to coated MDA-LDL in plasma of subjects homozygous for the H402 risk allele (CC, n = 38), heterozygous for the H402 risk allele (CT, n = 88) or homozygous for the Y402 allele (TT, n = 45). The association of rs1061170 with CFH binding to MDA was calculated with P = 1.29−40 using an additive model. Symbols represent individual subject samples with horizontal bars indicating the mean of each group. Values are mean ± s.d. RLU per 100 ms of triplicate determinations (***P < 0.001).