Figure 5. CFH neutralizes proinflammatory effects of MDA.

a, Expression of indicated genes in ARPE-19 cells stimulated for 24 h with 50 μg ml⁻¹ BSA compared to MAA-BSA as determined by quantitative RT–PCR. Data represent the mean ± s.e.m. of three independent experiments. b, Cell-based ELISA for the binding of biotinylated MAA-LDL to thioglycollate-elicited macrophages in the presence of BSA or CFH. Data are expressed as B/B₀ and represent mean ± s.d. of triplicate determinations. c, Secretion of IL-8 by THP-1 cells stimulated for 12 h with BSA or MAA-BSA in the absence or presence of CFH. Numbers below indicate concentrations of CFH, BSA and MAA-BSA in μg ml⁻¹. Error bars represent mean ± s.e.m. of three independent experiments. d, e, Intravitreal injection of BSA, MAA-BSA and/or CFH in mice (n = 4–5 per group). Six hours after injection, RPE/choroid was isolated. d, RT–PCR for Rpe65 and Rho in RPE/choroid fractions. cDNA isolated from neurosensory retina was used as a control (Ct). e, Expression of KC in RPE/choroid as assessed by quantitative RT–PCR. Error bars represent mean ± s.e.m. expression normalized to the BSA-injected group (*P < 0.05, **P < 0.01, ***P < 0.001).