Data Table 1. Data collection and refinement statistics (molecular replacement).
| Unmodified (4X62) |
m6A +1 (4X64) |
m6A +2 (4X65) |
m6A +3 (4X66) |
|
|---|---|---|---|---|
| Data collection | ||||
| Space group | P41212 | P41212 | P41212 | P41212 |
| Cell dimensions | ||||
| a, b, c (Å) | 400.8, 400.8, 175.1 | 400.5, 400.5, 175.5 | 401.4, 401.4, 175.9 | 401.3, 401.3, 175.6 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 |
| Resolution (Å) | 35.0-3.45 (3.51-3.45) | 35.0-3.35 (3.41-3.35) | 35.0-3.35 (3.41-3.35) | 55.0-3.45 (3.51-3.45) |
| Rmerge | 0.168 (0.984) | 0.130 (0.993) | 0.141 (0.929) | 0.210 (0.961) |
| I / σI | 15.10 (2.76) | 12.29 (1.25) | 19.08 (2.83) | 9.04 (1.77) |
| Completeness (%) | 99.2 (100.0 | 96.4 (91.4) | 100.0 (100.0) | 96.6 (98.7) |
| Redundancy | 10.5 (10.7) | 5,8 (2.7) | 11.9 (9.2) | 5.1 (4.9) |
| Refinement | ||||
| Resolution (Å) | 35.0-3.45 (3.49-3.45) | 35.0-3.35 (3.38-3.35) | 35.0-3.35 (3.38-3.35) | 50.0-3.45 (3.49-3.45) |
| No. reflections | 183968 (5548) | 171102 (1033) | 204105 (5914) | 180755 (5622) |
| Rwork / Rfree | 0.181/0.214 (0.272/0.296) | 0.165/0.204 (0.291/0.314) | 0.185/0.211) (0.261/0.303) | 0.194/0.226 (0.297/0.354) |
| No. atoms | ||||
| Protein | 19121 | 19121 | 19121 | 19121 |
| RNA | 32874 | 32875 | 32875 | 32875 |
| Ligand/Ion/Water | 782 | 829 | 844 | 826 |
| B factors | ||||
| Protein | 110.3 | 94.8 | 77.9 | 99.4 |
| RNA | 97.8 | 80.2 | 66.1 | 86.5 |
| Ligand/Ion/Water | 103.4 | 83.6 | 74.6 | 87.3 |
| r.m.s. deviations | ||||
| Bond lengths (Å) | 0.004 | 0.004 | 0.005 | 0.005 |
| Bond angles (°) | 0.790 | 0.859 | 0.819 | 0.801 |
One crystal was used for each dataset. aValues in parentheses are for highest-resolution shell.
[AU: Equations defining various R values are standard and hence are no longer defined in the footnotes.]
[AU: Ramachandran statistics should be in methods section at the end of the refinement sub-section.]
[AU: Wavelength of data collection, temperature, beamline should all be in methods section.]