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. Author manuscript; available in PMC: 2017 Jul 15.
Published in final edited form as: Dev Biol. 2015 Oct 9;415(2):251–260. doi: 10.1016/j.ydbio.2015.10.010

Fig. 5. Increased Runx2 expression and proliferation in the Bcl11b−/− embryonic faces and ectopic Runx2 expression within the Bcl11b−/− coronal suture.

Fig. 5

(A–H) RNA ISH using a Runx2 probe in sections of embryonic faces at E14.5 at two different planes (A–D) and at higher magnification (G, H) and in coronal suture at E16.5 (E, F). Arrowheads denote up-regulation and expansion of Runx2 expression. Asterisks indicate ectopic expression of Runx2 in Bcl11b−/− sutures. (I, J) BrdU immunostaining in sections of control and Bcl11bncc−/− heads at E14.5. Bones: F, frontal; M, maxillary; N, nasal; P, parietal; PM, premaxillary. Sutures: c, coronal. Scale bars: (A–D) 500 µm; (E, F) 200 µm; (G, J) 100 µm. (K) Quantification of the BrdU index (BrdU+ cells as a fraction of hematoxylin+ cells). Error bars indicate standard deviation. *** denotes statistical significance at p ≤ 0.001 (n = 3).