Skip to main content
. 2016 Mar 27;2016:1068528. doi: 10.1155/2016/1068528

Figure 5.

Figure 5

Hyperoside induces HSC apoptosis by blocking NF-κB activation and mediating NF-κB-dependent genes. (a) Hyperoside blocks NF-κB activation in Lx-2 cells. The effect of hyperoside on NF-κB DNA-binding activity in LX-2 cells was evaluated by ELISA. LX-2 cells were incubated for 48 hours in the absence or presence of TNF-α (20 ng/mL) and hyperoside (1 mM/L). Nuclear protein was extracted and subjected to ELISA for measurement of NF-κB DNA-binding activity. (b) Relative expression of NF-κB mediating proapoptotic target genes. (c) Relative expression of NF-κB mediating antiapoptotic target genes (∗means compared with the control group, P < 0.05; ∗∗means compared with the control group, P < 0.001, n = 3).