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PIO alleviated hypertrophy of cardiac myocytes in vitro. (a) The effects of PIO on viability of neonatal rat ventricular cardiomyocytes were determined by CCK-8 in 5 independent experiments. (b) The levels of ANP of cardiomyocytes in indicated groups (n = 5). (c) The immunofluorescence of cardiomyocytes and the sectional areas of myocytes (n = 5). ∗ P < 0.05.
