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Linear DNA loading into EVs by electroporation is size limited. HEK293T-derived EVs were electroporated in the presence of linear dsDNAs of the indicated sizes, and DNA loading was assessed after DNase I digestion via the PicoGreen assay. For all groups, n = 3, and all data were normalized to background signal generated by electroporated DNA only for each size. #P < 0.01 for each group compared to 1000 bp, 1500 bp, and 4000 bp groups.
