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. 2016 Feb 1;57(2):320–331. doi: 10.1167/iovs.15-18716

Figure 6.

Figure 6

Projection of CLSM z-stacks showing the distal muscle–tendon junction of MR muscles (A–D), a whole MR muscle (E) and a Golgi tendon organ (F) in lateral-eyed species. (A) and (B) illustrating only segments of the muscle–tendon junctions, whereas (C) and (D) the entire muscle-tendon junctions. Axons were labeled with anti-neurofilament (NF, red), nerve terminals with anti-synaptophysin (Syn, green), and muscle fibers with phalloidin (Phall, blue). Only in pig (A) and rabbit (B), are individual palisade endings (arrow) seen, and most axons stop at variable distances away from the muscle–tendon junction. Such axons establish numerous synaptophysin-positive contacts, indicating they supply multiply innervated muscle fibers. (A) A Golgi tendon organ (asterisk) is visible as well. In (C–E), all axons stop before reaching the muscle–tendon junction and no palisade endings are present. (E) Showing the nerve entry site (asterisk) and the motor endplate zone (arrow) in the proximal part of the muscle. (I) High magnification image of a Golgi tendon organ with synaptophysin-positive nerve terminals. Scale bars: (A, C) 200 μm; (B, D, E) 300 μm; (F) 50 μm.