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. 2016 Feb 1;57(2):320–331. doi: 10.1167/iovs.15-18716

Figure 8.

Figure 8

Projection of z-stacks from CLSM images showing palisade endings in lateral-eyed species. Palisade endings are from LR muscles in (A, B), and the rest from MR muscles. (A, C, E, G, I) Staining with anti-neurofilament (NF, red), anti-synaptophysin (Syn, green), and phalloidin (Phall, blue), and (B, D, F, H, J) with anti-neurofilament, α-bungarotoxin (α-BTX), and phalloidin. (A, C, E, G, I) Terminal varicosities in palisade endings exhibit synaptophysin immunoreactivity. In pig (A), sheep (C), and horse (E), terminal varicosities of palisade endings are found at the tendon level and also around the muscle fiber tip, whereas in rabbit (G) and rat (I), varicosities of palisade endings are only at the muscle fiber tip. (B, D, F) Alpha-bungarotoxin labeling is generally absent in palisade endings, but is found on neighboring muscle fibers ([B, F] arrows). However, in (H) and (J), α-bungarotoxin is present in association with palisade endings. Scale bars: (A–G) 50 μm; (H–J) 25 μm.