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. Author manuscript; available in PMC: 2016 Apr 10.
Published in final edited form as: J Vasc Res. 2009 Sep 4;47(2):93–107. doi: 10.1159/000235964

Fig. 7.

Fig. 7.

NONOate inhibits VGCC via a sGC-independent mechanism. a In isolated smooth muscle cells at 37°C under current-clamp conditions the resting Em oscillated. Addition of 1 µm NONOate (indicated by arrow) hyperpolarized the cell and abolished the oscillations in Em. b The voltage protocol for detecting ICaL (top) resulted in inward current that was reduced by 1 µm NONOate (bottom). c Mean current voltage relationships under both control conditions (n = 6, left) and after pretreatment with ODQ (n = 7, right) show that the inhibition of ICaL by 1 µm NONOate was not sensitive to ODQ. d The peak current was reduced by approximately 50% under both conditions. Data are expressed as means ± SEM. * p < 0.05, significant difference from control. e Effect of 1 µm NONOate (added at arrow) on peak ICaL amplitude over time, for data shown in c and d. NONOate-induced ICaL inhibition took minutes to occur, and was not due to current rundown (time control).