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. Author manuscript; available in PMC: 2017 Jun 1.

Published in final edited form as: Cell Signal. 2016 Feb 19;28(6):552–560. doi: 10.1016/j.cellsig.2016.02.010

Fig. 5 — Inosine induces mA_2AR-mediated intracellular cAMP production.

(A) CHO-K1 cells transiently transfected with mA_2AR but not with vector alone exhibit agonist-inducible cAMP production. Transiently transfected CHO-K1 cells were incubated with various agonists and an inverse agonist for 10 min. Mean intracellular cAMP levels ± SEM of representative experiments are shown (n=3; *, p < 0.05; **, p < 0.01 vs DMSO). Adenosine (Ado), inosine (Ino), A_2AR-selective agonist CGS 21680 (CGS) and A_2AR-selective inverse agonist ZM 241385 (ZM). Dose response of adenosine (B) and inosine (C) to mA_2AR. CHO-K1 cells transiently transfected with mA_2AR were incubated with various concentrations of adenosine and inosine. Mean cAMP levels ± SEM of a representative experiment in triplicates are shown. Shaded and dotted boxes indicate data with p < 0.001 and p < 0.05 relative to control, respectively. EC₅₀ values with 95% confidence intervals (in parentheses) were 79(21.5–293) nM and 773(249.5–2396) μM for adenosine and inosine respectively.

Fig. 5 — Inosine induces mA_2AR-mediated intracellular cAMP production.

(A) CHO-K1 cells transiently transfected with mA_2AR but not with vector alone exhibit agonist-inducible cAMP production. Transiently transfected CHO-K1 cells were incubated with various agonists and an inverse agonist for 10 min. Mean intracellular cAMP levels ± SEM of representative experiments are shown (n=3; *, p < 0.05; **, p < 0.01 vs DMSO). Adenosine (Ado), inosine (Ino), A_2AR-selective agonist CGS 21680 (CGS) and A_2AR-selective inverse agonist ZM 241385 (ZM). Dose response of adenosine (B) and inosine (C) to mA_2AR. CHO-K1 cells transiently transfected with mA_2AR were incubated with various concentrations of adenosine and inosine. Mean cAMP levels ± SEM of a representative experiment in triplicates are shown. Shaded and dotted boxes indicate data with p < 0.001 and p < 0.05 relative to control, respectively. EC₅₀ values with 95% confidence intervals (in parentheses) were 79(21.5–293) nM and 773(249.5–2396) μM for adenosine and inosine respectively.