Inosine dose-dependently induces hA2AR-mediated cAMP production in
cell-free membrane preparations.
CHO-hA2AR cell membranes were incubated with indicated concentrations
of CGS 21680 (CGS) and ZM 241385 (ZM) (A) and with inosine (B) in the presence
and in the absence of the A2AR-selective inverse agonist ZM 241385
for 30 min. Mean cAMP production ± SEM of representative experiments are
shown (n=6; * and ***, p < 0.05 and p < 0.001 vs DMSO
respectively; ## and ###, p < 0.001 and p < 0.001 vs inosine
respectively).