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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Dev Biol. 2016 Mar 2;412(2):219–233. doi: 10.1016/j.ydbio.2016.02.029

Fig. 10.

Fig. 10

Inhibitory effect of blebbistatin on elongation and coiling of Wolffian ducts. (A) Decreased elongation and coiling when E16.5 wild-type Wolffian ducts were incubated with 2.5 µM or 5 µM blebbistatin compared to DMSO controls. The images were taken at 0h, 5h, and 24h. After 24h culture, blebbistatin was removed, and the images were recorded after 24h of inhibitor withdrawal. (B) Statistical analysis of elongation and coiling of Wolffian ducts during the first 24h of incubation with blebbistatin. n ≥ 3. Data are presented as mean ± SEM. ** p<0.01.