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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Dev Biol. 2016 Mar 2;412(2):219–233. doi: 10.1016/j.ydbio.2016.02.029

Fig. 11.

Fig. 11

Inhibitory effects of Y27632 on elongation and coiling of Wolffian ducts. (A) Decreased elongation and coiling when E16.5 wild-type Wolffian ducts were incubated with 1µM or 2.5µM Y27632 compared to DMSO controls. The images were recorded at 0h, 24h, and 48h. Y27632 was then removed, and the images were taken after 24h of inhibitor withdrawal. (B) Statistical analysis of elongation and coiling of Wolffian ducts during the first 24h of incubation with Y27632. A curve, which has an angle less than 60 degree, is considered as a bend. n ≥ 3. Data are presented as mean ± SEM. ** p<0.01.