Changes of PTK7 localization during Wolffian duct development. Representative images show PTK7 immunolocalization in control proximal Wolffian ducts at E18.5 (A), P7 (B), P14 (C), adults (D), and in TCre-cKO proximal Wolffian ducts at E18.5 (E) and adults (F), respectively. Yellow arrows show epithelial cells. Blue arrowheads show mesenchymal cells surrounding the epithelium. The white arrow points to punctate labeling from the gene trap mutant Ptk7 allele (Xst87).