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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Virology. 2016 Feb 23;492:82–91. doi: 10.1016/j.virol.2016.02.012

FIG. 6.

FIG. 6

The IE62 dimerization domain influences transactivation in vitro. Results of triplicate transient transfection assays determining the effect of IE62 dimerization domain mutations on IE62 mediated transactivation are shown for A) the ORF29 promoter, B) the ORF62 promoter, and C) the TA-Luc model promoter. Luciferase activities in the absence of IE62 were normalized to one. Promoter activities in the presence of IE62 are reported as induction (n-fold) of the luciferase activity over the basal activity. Statistical significance was determined by a one-way ANOVA analysis of variance followed by Tukey’s post hoc test. D) Western blot analyses show expression levels of IE62, and β-tubulin. β-tubulin was used as loading control in experiments.