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. 2016 Apr 11;10:96. doi: 10.3389/fncel.2016.00096

Table 2.

Primer sequences and restriction enzymes used for detection of the investigated polymorphisms.

Gene polymorphism (alleles) PCR primers (5′−3′) Enzyme (fragment bp)
rs1467967 (G/A) Fwr: CACAGCCACCCTCCCTCTAAC DraI
Rev: GGCTCCACCCTTCAGTTTTGGA (267/ 186, 81)
rs242557 (A/G) Fwr: CTTGATGATGCATGGACCTCTC ApaHI
Rev: TTGACAGTACCCACGACACGTG (211/ 139, 72)
rs3785883 (A/G) Fwr: CCATCACCTTGTCAGAAACTC BsaHI
Rev: AGCCATGTGGTAGCCTCAG (277/ 164, 113)
rs2471738 (T/C) Fwr: CTCTCTGGACCCTCATCCACC BstEII
Rev: GAGAACCGAATGAGGACTGGAA (170/ 104, 66)
rs7521 (G/A) Fwr: ACCTCTGTGCCACCTCTCAC PstI
Rev: AGGTGAGGCTCTAGGCCAGT (231/ 160, 71)

Fwr, forward primer; Rev, reverse primer.