Table 9.
Genes and qRT-PCR primers evaluated in this study.
| Clan | Family | MEROPS ID | Peptidase sp. | Cluster | Primer sequences (5′- 3′) | L (bp)a | E (%)b | R 2 c |
|---|---|---|---|---|---|---|---|---|
| AA | A1 | A01.092 |
CAD2 peptidase (M. domestica) |
LST_LS005916 | 5′-GCTGCCAAGCTATTGCTGAT-3′ | 75 | 102 | 0.997 |
| 5′-CGGCTTGAATGTTTTCGTATT-3′ | ||||||||
|
| ||||||||
| CA | C1 | C01.A27 |
CG12163 protein (D. melanogaster) |
LST_LS006048 | 5′- TTTCACCGGTAATCGCAAAT -3′ | 66 | 105 | 0.997 |
| 5′- GCAGCCTTTTGACGATGTTT -3′ | ||||||||
|
| ||||||||
| MA | M1 | M01.024 | ERAP2 aminopeptidase | LST_LS004632 | 5′- CCATTCCGTCCGAT TCTT -3′ | 68 | 98 | 0.999 |
| 5′- ATCGGCATACTGGGCAAA -3′ | ||||||||
|
| ||||||||
| PB | T1 | T01.976 | Proteasome subunit alpha 1 | LST_LS006843 | 5′- TCTTCACGCTCTCAAGACGA -3′ | 61 | 103 | 0.993 |
| 5′- GGTCTTGTGTTTCGCCATCT -3′ | ||||||||
|
| ||||||||
| PA | S1 | S01.993 |
Testis-specific protein 50 (H. sapiens) |
LST_LS010066 | 5′- TATGGGCAGCCCTAACGA -3′ | 60 | 104 | 0.998 |
| 5′- AAAGAACGGAGAGCATCACCT -3′ | ||||||||
aLength of amplicon.
bQuantitative qRT-PCR efficiency.
cCoefficient of determination.