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. 2016 Apr 11;6:24290. doi: 10.1038/srep24290

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HA levels in the cell lysates were detected by Western blotting analysis and no band was observed in the control (β-actin), which confirmed successful expression of the target antigenic protein HA. HA and β-actin were 64 KD and 42 KD, respectively. BV-S series (BV-S-HA, BV-S-ITRs-HA and BV-S-con-HA) refers to the recombinant baculovirus start by CMV, containing different regulatory elements of BV-S-HA (WPRE), BV-S-ITRs-HA (WPRE, ITRs), BV-S-con-HA; BV-A series (BV-A-HA, BV-A-ITRs-HA and BV-A-con-HA) refers to the recombinant baculovirus start by ie1, containing different regulatory elements of BV-A-HA (WPRE), BV-A-ITRs-HA (WPRE, ITRs), BV-A-con-HA.