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(a) Diagram of Cas9-mediated knock-in of LA-CAG-tGFP-polyA-RA into the sRosa26 locus. LA, left arm (1.0 kb); RA, right arm (1.0 kb). (b) CAG promoter-driven tGFP expression in sheep fibroblasts (CAG promoter region is shown in supplementary information).
