Figure 2. Effect of EutJ, EutP and EutQ expression on EutSMNLK nanocompartment assembly in E. coli.

The left side of the panel displays cells co-expressing the shell proteins EutSMNLK along with EutJ, P, or Q and an untagged EGFP reporter. Images on the right side show cells co-expressing EutSMNLK along with EutJ, P, or Q and the EutC^1–19-EGFP fusion. Control cells (Empty: expressing an empty plasmid without eut genes) expressing cargo proteins alone are also shown for comparison. Arrowheads indicate the presence of EGFP foci. DIC images are shown to highlight the cell boundaries. The scale bar represents 1 μm. Representative crops of images are displayed, additional images are provided in Supplementary Figure 5.