Figure 7. EutQ and EutM interact in vitro.

(A) Green or red shaded boxes indicate a positive or negative interaction, respectively, as assessed by affinity-based pull-downs of untagged Eut shell proteins. (B) SDS-PAGE analysis of protein pulldowns between C-terminally His₆ (CTH6) tagged EutQ and EutM. The first lane demonstrates the co-elution of a low level of both proteins at a low imidazole concentration (50 mM), while the second lane demonstrates co-elution of both proteins at a high imidazole concentration (250 mM). (C) SDS-PAGE analysis of protein pulldowns between C-terminally His₆ (CTH6) tagged EutM and an N-terminally truncated version of EutQ (∆EutQ1–100). The first lane demonstrates the elution of a single protein at a low imidazole concentration (50 mM), while the second lane demonstrates the elution of a single protein at a high imidazole concentration (250 mM). The identity of the protein in these bands was confirmed as EutM-CTH6 by peptide mass sequencing (Supplementary Figure 13).