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. 2015 Oct 15;18(5):679–690. doi: 10.1093/neuonc/nov248

Fig. 1.

Fig. 1.

Extracellular expression of CD15 in brain endothelial and lung cancer cell lines. (A) Representative immunocytochemical images showing extracellular expression of CD15 in human brain endothelial cells (hCMEC/D3), human non–small cell lung cancer cells (NSCLC) metastatic cells obtained from cervical lymph node (NCI-H1299), brain (SEBTA-001 and SEBTA-005) and in nonmetastatic NSCLC cells (A549 and COR-L105). (B) Semi-quantitative analysis of CD15 expression from confocal images (A) using Zeiss ZEN image software. (C) Representative flow cytometric histogram. (D) Flow cytometric analysis of CD15 expression on hCMEC/D3, NCI-H1299, SEBTA-001, SEBTA-005, A549, and COR-L105. CD15 was highly expressed on NCI-H1299 and SEBTA-001 with less expression on COR-L105 and SEBTA-005, which expressed relatively the same amount. N = 3, ***P < .0001, **P < .001 and *P < .01. There was also less CD15 expression on A549 and hCMEC/D3 cells. (E) Western blot of proteins from the cell lines showed highest CD15 expression in NCI-H1299, followed by SEBTA-001, SEBTA-005, COR-L105, A549, and hCMEC/D3. ABCE1 was used as a protein loading control.