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. 2016 Apr 11;6:24104. doi: 10.1038/srep24104

Figure 4. Pk3 depletion prevents γ-tubulin and Nedd1 recruitment to the basal body.

Figure 4

Embryos were injected with 0.2 ng of RNA encoding GFP (A–D) or Histone-GFP (E,F) as a lineage tracer and CoMO (A,C,E) or Pk3MO1 (B,D,F) (15 ng each). GRP explants from stage 17 embryos were double stained for GFP and γ-tubulin (A,B), Nedd1 (C,D) or Centrin1 (E,F). En face staining is shown, anterior is at the top. Representative images from three to five independent experiments are shown, with 6–10 explants examined per group. The effects of Pk3MO1 on γ-tubulin and Nedd1 are detectable in the majority of cells depleted of Pk3 (n > 100).