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. 2016 Apr 11;6:24212. doi: 10.1038/srep24212

Figure 1. Correlation of amide-conjugates IAA-Ala, IAA-Leu and IAA-Phe with TIR1/AFBs signaling pathway using ratiometric auxin sensor in plant cells.

Figure 1

(a,c,d) Sensitivity of the auxin sensor towards IAA-Ala, IAA-Leu, IAA-Phe and IAA, in plant cells. Protoplasts from Col-0 wild type (a) ilr1ill2iar3 triple mutant (c) or both (d) were transformed with the sensor construct. After 20 h incubation in hormone-free medium, 0, 1, 5, 10, 20, 30 μM each of IAA-Ala, IAA-Leu and IAA-Phe (a,c) or 0, 0.1, 1, 10 μM IAA (d) were added to the samples. After 1 h treatment, the ratiometric determinations of firefly/renilla (F/R) were performed. Results are represented as percentile of the control samples (no auxin treatment). Results are means ± s.e.m. (n = 16). Statistical significances for each conjugates are indicated with lower case letters (the same letters indicate no statistical significant difference between the samples, one-way ANOVA, P < 0.001). (b) Expression analysis of ILL1, ILL2, ILL3, IAR3, ILL5, ILL6 and ILR1 in Arabidopsis Col-0 protoplast-derived cells. ACTIN (ACT) was included as a positive control.