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. 2016 Apr 11;6:24212. doi: 10.1038/srep24212

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The sensor was co-expressed with either ILR1, or ILL2 or IAR3 in triple mutant ilr1ill2iar3 protoplasts. GFP was also co-expressed as a control. After 20 h incubation in hormone-free medium, 0, 1, 5, 10, 20, 30 μM each of IAA-Ala, IAA-Leu and IAA-Phe were supplemented to the culture medium. After 1 h treatment, the ratiometric determinations (F/R) were performed. Results are represented as percentile of the control samples (no auxin treatment). Results are means ± s.e.m. (n = 16).