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. Author manuscript; available in PMC: 2016 Apr 11.
Published in final edited form as: Biomaterials. 2015 Jun 20;65:93–102. doi: 10.1016/j.biomaterials.2015.06.036

Fig. 4.

Fig. 4

Capture and release of spiked PC-3 cells. (a) Left: optical image of whole blood flowing through an HBCTC-chip. The concentration of spiked PC-3 cells in whole blood was 5000/mL. Right: image shows a representative distribution of captured cells (shown as black dots) in the HBCTC-chip. The scale bar is 10 mm. (b) Capture efficiency of ALG/PAH modified HBCTC-chip in comparison with conventional HBCTC-chip. (c) Release efficiency at various degradation times and flow rates of enzyme solution. (d)–(f) Immunofluorescent staining of cell surface receptors for captured PC-3 cells in the HBCTC-chip (d), released PC-3 cells (e), and white blood cells (f), shown here is EpCAM expression in green, DAPI nuclear staining in blue, and CD45 expression in red. The scale bars are 20μm.