Fig. 8.

Rab5a regulates LPS-induced HPMEC dysfunction. a HPMECs were transfected with NC siRNA or Rab5a siRNA for 48 h and then stimulated with 1 μg/ml LPS for 6 h. Real-time cell growth curves were generated using the iCELLigence system. The data are shown as the mean ± SE from three independent experiments. b Quantification of the normalized CI in the histogram. After transfection with NC siRNA and Rab5a siRNA, the cells were stimulated with LPS for 6 h. The data are presented as the mean ± SE, n = 3. *p < 0.05 versus the control group; ^p < 0.05 versus the NC siRNA plus LPS treatment group. c HPMECs were cultured on 0.4-μm Transwell inserts and transfected with NC siRNA or Rab5a siRNA for 48 h and then stimulated with 1 μg/ml LPS for 6 h. FITC-dextran was added to the upper wells at a concentration of 1 mg/ml. After 0.5 h of incubation, 50 μl of medium from the bottom chamber was aspirated and analyzed using a fluorescence plate reader. FITC-dextran permeability was expressed as fold increase ± SE (n = 3). *p < 0.05 versus the control group; ^p < 0.05 versus the NC siRNA plus LPS treatment group