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. Author manuscript; available in PMC: 2016 Apr 11.
Published in final edited form as: Science. 2012 Feb 3;335(6068):590–593. doi: 10.1126/science.1212867

Figure 3. Notch down regulates nuclear TCF in mesoderm.

Figure 3

(A to C) Nuclear TCF clears from mesoderm (brackets; SoxB1-negative; see fig. S9 for lineage identification using SoxB1) in control embryos. (D to F) Without Notch or (G to I) NLK, nuclear TCF persists in inner veg2 descendants (brackets). (J) Summary of endomesoderm segregation steps. (Step 1) Notch inhibits the Hox11/13b regulatory circuit in mesoderm (green box) by HB stage to initiate segregation. The Hox11/13b circuit stays active in endoderm (yellow). (Step 2) Consequently, by MB stage, Hox11/13b- and Brachyury-dependent expression of wnt1 is restricted to endoderm, where it reinforces cWnt signaling and endoderm fate. (Step 3) By pregastrula stage, a Notch-NLK pathway clears nuclear TCF from mesoderm, making it unresponsive to cWnt. The endoderm retains nuclear TCF and cWnt sensitivity.