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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Dev Biol. 2015 Nov 11;409(1):261–271. doi: 10.1016/j.ydbio.2015.10.031

Fig. 2.

Fig. 2

Mitotic index and DNA content in wild type, glp-1, daf-2, and rsks-1 larval and adult stages (A): Mitotic Index of wild type, glp-1(e2141), daf-2(e1370) and rsks-1(sv31) mutants at larval (mid-L4) and adult stages at 20 °C. Number of gonad arms (N) and nuclei (n) for larval: wild type, N = 22, n = 2989; glp-1, N = 69, n = 4610; daf-2, N = 15, n = 2279; rsks-1, N = 15, n = 1121. For adult: wild type, N = 21, n = 4014; glp-1, N = 32, n = 3193; daf-2, N = 22, n = 3047; rsks-1, N = 20, n = 2141. (B): Pooled average DNA content of wild type, glp-1(e2141), daf-2(e1370) and rsks-1(sv31) mutants, in larval and adult stages. Statistics: two-tailed Mann–Whitney U test, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 and n.s. indicates not significant. Note, pooled average DNA content of daf-2(e1370) and rsks-1(sv31) adults is not significantly different from wild type adult (p > 0.05). (C): Pooled average DNA content sub-divided into Low, Mid and High DNA content bins (see Materials and methods). Y-axis is the proportion of nuclei measured within the different DNA content bins. For larval, wild type, N = 27, n = 1524; glp-1(e2141), N = 56, n = 2090; daf-2(e1370), N = 44, n = 2487; rsks-1(sv31), N = 33, n = 1667. For adult: wild type, N = 26, n = 1940; glp-1(e2141), N = 34, n = 1899; daf-2(e1370), N = 33, n = 2530; rsks-1(sv31), N = 35, n = 3157.