Fig. 3.

Summary of average DNA content across the proliferative zone. In all graphs, the Y-axis is the average percent of nuclei measured in Low, Mid and High DNA content bins (see Materials and methods) and the X-axis is the distance from the distal tip in cell diameters (grouped in increments of 5). Graphs on the left side (A–D) are larval and on the right side (A’-D’) are adult, both at 20 °C. Number of animals (N) and nuclei (n) are as follows: wild type: larval N = 27, n = 1524, adult N = 26, n = 1940; glp-1(e2141): larval N = 56, n = 2090, adult N = 34, n = 1899; daf-2(e1370): larval N = 44, n = 2487, adult N = 33, n = 2530; rsks-1(sv31): larval N = 33, n = 1667, adult N = 35, n = 3157 (see also Table S1). Statistics: mixed effect regression models (see Materials and methods) were used to determine effects of distance from the distal tip, age and genotype on DNA content. Note that the data are averaged and binned in this graphical representation, but the mixed effects regression model uses all observations. Region “CD ≤ 5” was used as reference to compute p-values for the remaining cell diameter regions in each genotype. **0.05 ≥ p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001 and n.s. is not significant.