FIG. 4.

Culture and proliferation of SUM159-GFP breast cancer cells on methagel/PEGDA blended self-folded tubes. SUM159-GFP cells were seeded onto the surface of hydrogel blend bilayers with the outer layer made of methagel PEGDA and the inner layer of PEGDA 700. Epifluorescence images of GFP-positive cells (green) were taken over a series of days. (A) By day 2, cells that had attached grew sparsely. (B, C) Cells proliferated and began filling in the empty spaces along the hydrogel surface during day 4 and 6. (D) By day 9, cells were densely populated and the hydrogel tube was overgrown. Scale bar is 200 μm and corresponds to 100 μm in insets. GFP, green fluorescent protein. Color images available online at www.liebertpub.com/tec