FIG. 3.

Immunohistochemical staining with Iba-1 antibody at 7 days after second sham insult or mild traumatic brain injury (mTBI). Post-natal day (PND) 18 rats were divided into three groups and subjected to (1) two sham insults (S-S), (2) one mTBI, one sham insult (mTBI-S), or (3) two mTBIs (mTBI-mTBI) 24 h apart. (A) Representative views of coronal sections (scale bar = 200 μm) at approximately −3.0 to 4.5 mm, bregma. Insets include higher magnification (scale bar = 25 μm) image of Iba-1 immunoreactive microglia indicated by arrows. (B) Red (cortex), blue (external capsule), and black (amygdala) boxes indicate areas of interest, shown in higher magnification in panel A. (C) Quantification of the number of Iba-1 immunoreactive cells in PND 18 brains (n = 3/group). Mean ± standard error of the mean. Color image is available online at www.liebertpub.com/neu