Regions of interest (ROIs) were selected in a number of brain areas on the anatomical T1-weighted rapid acquisition with relaxation enhancement (RARE) images (A) acquired after exposure to blast and the administration of the MnCl2 contrast agent. The mean intensity in the gray scale was determined for every ROI at 6, 24, 48, 72 h, and 14 and 28 days after injury. A schematic representation of all ROIs (B) are depicted for caudate putamen, median preoptic nucleus, anterior nucleus hypothalamus, cortex, hippocampus-CA1/CA3/DG, hippocampus-CA1, cerebellum, medial vestibular nucleus, lateral superior olivary nucleus, and principal sensory trigeminal nucleus.