FIG. 2.

Candida outgrowth in the liver and kidneys of mice during systemic infection. Candida colony-forming units (CFUs) from liver (A) and kidneys (B) from wild-type and Dectin-2^−/− mice at day 3, 7, and 14 postinfection. Wild-type and Dectin-2^−/− mice received 1 × 10⁵ CFU live C. albicans (per mouse), intravenously. Results are presented as mean ± SEM (n = 9–12 mice per group from 3 independent experiments). Significance was determined with Mann–Whitney U-test. Statistically different groups are indicated as *P < 0.05; **P < 0.01; ***P < 0.001. Histopathological assessment of kidneys on day 14 in uninfected Dectin-2^−/− (C, 100×), infected wild-type (D,2.5×), infected Dectin-2^−/− (E, 2.5×) (hematoxylin and eosin-stained sections) and infected Dectin-2^−/− (F,200×, and G, 400×) mice (periodic acid–Schiff-stained sections). Representative localized lesions and C. albicans colonies are shown in the kidneys of Dectin-2^−/− mice (D, E). The dimension of inflammatory foci is represented in (H). A minimum of 20 foci were measured in at least 7 different kidneys. Results are mean ± SEM (n = 4 mice per group) from 2 independent experiments. Significance was determined with Mann–Whitney U-test. Statistically different groups are indicated as ***P < 0.001.