FIG. 4.

Stimulation of cells isolated from wild-type mice with C. albicans mutants. C. albicans mutants lacking O- and N-linked, α- and β-mannan were screened for their capacity to induce cytokines in wild-type peritoneal macrophages (A) and splenocytes (B) from naïve mice. Values represent mean ± SEM (n = 8 mice per group, 2 independent experiments). Significance was determined with Mann–Whitney U-test. Statistically different groups are indicated as *P < 0.05; **P < 0.01; ***P < 0.001. Ca hy, C. albicans hyphae heat-killed; Ca y, C. albicans yeast heat-killed. The control strain NGY152, was derived from CAI-4, which is the parental Ura-auxotrophic strain of all mutants analyzed, and was created by reintegration of a copy of URA3 gene at the RPS1 locus.