FIG. 6.

Injectibility of hMSC spheroids cultured under low and normal oxygen conditions tested using needles with different gauge sizes, including 18, 23, and 26 gauges. The F-actin (red) and cell nuclei (blue) were stained to observe the spheroid morphology (A). hMSC spheroids appeared undamaged after injection conditions. The DNA assay showed that there was no significant difference in the DNA content among all the culture conditions (B). Color images available online at www.liebertpub.com/tec