Figure 4.

Predicted rejection risk scores calculated from the pretransplant baseline SVM model. (A) The SVM was constructed using only baseline data (at or immediately before transplantation) and risk scores for rejection [0,1] were derived from the model as described in Section “Materials and Methods.” A score of >0.5 is at risk of rejection. Open triangles are samples from patients with rejection episodes, and filled cycles from patients without rejection. (B) The SVM was consequently tested on a further cohort of five patients who were analyzed by flow cytometry by a scientist not involved in the initial studies. As control, one patient who was previously analyzed was re-tested in this independent analysis. None of these patients experienced rejection episodes. The SVM trained on the initial patients correctly predicts all patients in this validation analysis to be at low risk of rejection.