Figure 5.

Baseline frequencies and flow cytometry profiles of the subsets with largest coefficients in the SVM. (A) Relative frequencies (% of parent population) of the five T cell subsets with largest SVM coefficients. The result for each patient is shown as a circle (red for rejectors, black for non-rejectors). Significance values (Mann–Whitney with Bonferroni correction) for the five subsets are: PD1⁺ naive Treg (CD4⁺ CD25⁺,CD127⁻, CD45RO⁻/CD62L⁺, PD1⁺) p = 0.02, CD25⁺ effector memory CD8⁺ T cells (CD8⁺, CD45RO⁺/CD62L⁻, CD25⁺) p > 0.1, PD1⁺ effector memory CD8⁺ T cells (CD8⁺, CD45RO⁺/CD62L⁻, PD1⁺) p > 0.1, PD1⁺ end-stage Treg (CD4⁺ CD25⁺,CD127⁻, CD45RO⁻/CD62L⁻, PD1⁺) p = 0.1, and CD127⁺ effector memory CD4⁺ T cells (CD4⁺, CD45RO⁺/CD62L⁻, CD127⁺) p > 0.1. (B) Dotplots showing PD1 (y axis) and FSC (x axis) expression in the five T cell subsets are described in (A), in three representative patients that did not reject, and three representative patients that did reject.